Recombinant:Article Title: CRIPTO-based micro-heterogeneity of mouse muscle satellite cells enables adaptive response to regenerative microenvironment.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Cripto Allophycocyanin MAb Biotechne - R&D systems Cat# FAB1538A Cripto Mouse specific Cell Signaling Technology Cat# 2818; RRID: AB_2240508 Anti-P4HB antibody [RL90] abcam Cat# ab2792; RRID: AB_303304 Pax7 Antibody Developmental Studies Hybridoma Bank (DHSB) Cat# PAX7; RRID: AB_528428 Anti-MyoD (C-20) Santa Cruz Biotechnology, Inc Cat# sc-304; RRID: AB_631992 Anti-GFP antibody abcam Cat# ab13970; RRID: AB_300798 Anti-human Ki67 Monosan Cat# MONX10283 Myogenin Antibody (F5D) Developmental Studies Hybridoma Bank (DHSB) Cat# F5D, RRID: AB_2146602 BrdU antibody [BU1/75 (ICR1)] GeneTex Cat# GTX26326; RRID: AB_1081056 Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6) ThermoFisher Scientific Cat# MA5-14520; RRID: AB_10979488 Anti-Laminin Sigma-Aldrich L9393; RRID: AB_477163 Biotin-SP (long spacer) AffiniPure Goat Anti-Mouse IgG, Fcg subclass 1 specific Jackson ImmunoResearch Cat# 115-065-205, RRID: AB_2338571 Anti-mouse Alexa Fluor 549 ThermoFisher Scientific Cat# A-21203; RRID: AB_2535789 Anti-rabbit Alexa Fluor 488 ThermoFisher Scientific Cat# A-11001; RRID: AB_2534069 Anti-rabbit Alexa Fluor 555 ThermoFisher Scientific Cat# A-31572; RRID: AB_162543 Anti-rabbit Alexa Fluor 549 ThermoFisher Scientific Cat# A-21207; RRID: AB_141637 Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) ThermoFisher Scientific Cat# 703-545-155, RRID: AB_2340375 Cy 3 AffiniPure Donkey Anti-Rat IgG (H+L) Jackson ImmunoResearch Cat# 712-165-153, RRID: AB_2340667 Chemicals, peptides, and recombinant proteins Sambucus Nigra Lectin (SNA, EBL), Biotinylated Vector Laboratories Cat# B-1305-2 Rhodamine Phalloidin ThermoFisher Scientific Cat# R415 Streptavidin-Cy3 conjugated Jackson ImmunoResearch Cat# 016-160-084 Streptavidin-488 conjugated Invitrogen Cat# S32354 Streptavidin-647 conjugated Invitrogen Cat# 532357 Tamoxifene Sigma-Aldrich Cat# T5648 Cardiotoxin Latoxan Laboratory Cat# L8102 Dispase II Roche Cat# 10887800 Collagenase A Roche Cat# 10103586001 DNAse I Roche Cat# 11284932001 Hank’s Balanced Salt Solution (HBSS) Gibco Cat# 14170-088 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A4503 Penicillin-Streptomycin Gibco Cat# 15140-122 Collagenase Sigma-Aldrich Cat# C0130 Dulbecco’s Modified Eagle’s medium (DMEM) Gibco Cat# 41965-039 Ham’s F-12 Nutrient Mix, GlutaMAX Gibco Cat# 31765-027 Pyruvate Sodium Gibco Cat# 11360-039 L-Glutamine 200mM (100x) Gibco Cat# 25030-024 (Continued on next page) e1 Developmental Cell 58, 2896–2913.e1–e6, December 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal Bovine Serum (FBS) Euroclone Cat# ECS5000L Ultroser G Sartorius Cat# 15950-017 Goat Serum Merck Cat# G9023 Nocodazole Sigma-Aldrich Cat# M1404 U73122 Selleckchem Cat# S8011 Varespladib (LY315920) Selleckchem Cat# S1110 FIPI Selleckchem Cat# S7321 Trizol Reagent Invitrogen Cat# 15596018 SYBR Green PCR master mix (FluoCycle II SYBR EuroClone Cat#ERD002250BIM BD Matrigel Basement Membrane Matrix BD Biosciences Cat# 354234 5-bromo-2’deossiuridina (BrdU) Sigma-Aldrich Cat# B5002 Bovine Serum Albumin (IgG Free, Protease Free) Jackson ImmunoResearch Cat# 001-000-162 AffiniPure Fab Fragment goat anti-mouse IgG Jackson ImmunoResearch Cat# 115-007-003 FluorSave Reagent Calbiochem Cat# 345789 Critical commercial assays MitoTracker Orange CM-H2TMRos ThermoFisher Scientific Cat# M7511 Wizard Genomic DNA Purification kit Promega Cat# A1120 MethylFlashTM Global DNA Hydroxymethylation (5hmC) ELISA Easy Kit Epigentek Cat# P-1032-96 Direct-Zol Microprep kit Zimo-Research Cat# R2060 QuantiTect Reverse Transcription Kit Qiagen Cat# 205311 Click-iT EdU Alexa Fluor 647 imaging kit Invitrogen Cat# C10340 Deposited data Raw RNA-seq data This paper SRA: PRJNA637587 Experimental models: Organisms/strains Mouse: Tg:Pax7-nGFP The Jackson Laboratories MGI:5308730 Mouse: C57BL/6 Charles River C57BL/6 mice Mouse: Tg:Pax7-nGFP::R26mTmG This paper N.A.
Modification:Article Title: CRIPTO-based micro-heterogeneity of mouse muscle satellite cells enables adaptive response to regenerative microenvironment.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse Cripto Allophycocyanin MAb Biotechne - R&D systems Cat# FAB1538A Cripto Mouse specific Cell Signaling Technology Cat# 2818; RRID: AB_2240508 Anti-P4HB antibody [RL90] abcam Cat# ab2792; RRID: AB_303304 Pax7 Antibody Developmental Studies Hybridoma Bank (DHSB) Cat# PAX7; RRID: AB_528428 Anti-MyoD (C-20) Santa Cruz Biotechnology, Inc Cat# sc-304; RRID: AB_631992 Anti-GFP antibody abcam Cat# ab13970; RRID: AB_300798 Anti-human Ki67 Monosan Cat# MONX10283 Myogenin Antibody (F5D) Developmental Studies Hybridoma Bank (DHSB) Cat# F5D, RRID: AB_2146602 BrdU antibody [BU1/75 (ICR1)] GeneTex Cat# GTX26326; RRID: AB_1081056 Ki-67 Recombinant Rabbit Monoclonal Antibody (SP6) ThermoFisher Scientific Cat# MA5-14520; RRID: AB_10979488 Anti-Laminin Sigma-Aldrich L9393; RRID: AB_477163 Biotin-SP (long spacer) AffiniPure Goat Anti-Mouse IgG, Fcg subclass 1 specific Jackson ImmunoResearch Cat# 115-065-205, RRID: AB_2338571 Anti-mouse Alexa Fluor 549 ThermoFisher Scientific Cat# A-21203; RRID: AB_2535789 Anti-rabbit Alexa Fluor 488 ThermoFisher Scientific Cat# A-11001; RRID: AB_2534069 Anti-rabbit Alexa Fluor 555 ThermoFisher Scientific Cat# A-31572; RRID: AB_162543 Anti-rabbit Alexa Fluor 549 ThermoFisher Scientific Cat# A-21207; RRID: AB_141637 Alexa Fluor 488 AffiniPure Donkey Anti-Chicken IgY (IgG) (H+L) ThermoFisher Scientific Cat# 703-545-155, RRID: AB_2340375 Cy 3 AffiniPure Donkey Anti-Rat IgG (H+L) Jackson ImmunoResearch Cat# 712-165-153, RRID: AB_2340667 Chemicals, peptides, and recombinant proteins Sambucus Nigra Lectin (SNA, EBL), Biotinylated Vector Laboratories Cat# B-1305-2 Rhodamine Phalloidin ThermoFisher Scientific Cat# R415 Streptavidin-Cy3 conjugated Jackson ImmunoResearch Cat# 016-160-084 Streptavidin-488 conjugated Invitrogen Cat# S32354 Streptavidin-647 conjugated Invitrogen Cat# 532357 Tamoxifene Sigma-Aldrich Cat# T5648 Cardiotoxin Latoxan Laboratory Cat# L8102 Dispase II Roche Cat# 10887800 Collagenase A Roche Cat# 10103586001 DNAse I Roche Cat# 11284932001 Hank’s Balanced Salt Solution (HBSS) Gibco Cat# 14170-088 Bovine Serum Albumin (BSA) Sigma-Aldrich Cat# A4503 Penicillin-Streptomycin Gibco Cat# 15140-122 Collagenase Sigma-Aldrich Cat# C0130 Dulbecco’s Modified Eagle’s medium (DMEM) Gibco Cat# 41965-039 Ham’s F-12 Nutrient Mix, GlutaMAX Gibco Cat# 31765-027 Pyruvate Sodium Gibco Cat# 11360-039 L-Glutamine 200mM (100x) Gibco Cat# 25030-024 (Continued on next page) e1 Developmental Cell 58, 2896–2913.e1–e6, December 18, 2023 .. REAGENT or RESOURCE SOURCE IDENTIFIER Fetal Bovine Serum (FBS) Euroclone Cat# ECS5000L Ultroser G Sartorius Cat# 15950-017 Goat Serum Merck Cat# G9023 Nocodazole Sigma-Aldrich Cat# M1404 U73122 Selleckchem Cat# S8011 Varespladib (LY315920) Selleckchem Cat# S1110 FIPI Selleckchem Cat# S7321 Trizol Reagent Invitrogen Cat# 15596018 SYBR Green PCR master mix (FluoCycle II SYBR EuroClone Cat#ERD002250BIM BD Matrigel Basement Membrane Matrix BD Biosciences Cat# 354234 5-bromo-2’deossiuridina (BrdU) Sigma-Aldrich Cat# B5002 Bovine Serum Albumin (IgG Free, Protease Free) Jackson ImmunoResearch Cat# 001-000-162 AffiniPure Fab Fragment goat anti-mouse IgG Jackson ImmunoResearch Cat# 115-007-003 FluorSave Reagent Calbiochem Cat# 345789 Critical commercial assays MitoTracker Orange CM-H2TMRos ThermoFisher Scientific Cat# M7511 Wizard Genomic DNA Purification kit Promega Cat# A1120 MethylFlashTM Global DNA Hydroxymethylation (5hmC) ELISA Easy Kit Epigentek Cat# P-1032-96 Direct-Zol Microprep kit Zimo-Research Cat# R2060 QuantiTect Reverse Transcription Kit Qiagen Cat# 205311 Click-iT EdU Alexa Fluor 647 imaging kit Invitrogen Cat# C10340 Deposited data Raw RNA-seq data This paper SRA: PRJNA637587 Experimental models: Organisms/strains Mouse: Tg:Pax7-nGFP The Jackson Laboratories MGI:5308730 Mouse: C57BL/6 Charles River C57BL/6 mice Mouse: Tg:Pax7-nGFP::R26mTmG This paper N.A.
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